iSeq 100 System
iSeq 100
Next-generation sequencing (NGS) is easier and more affordable with the compact Illumina iSeq 100 System. The iSeq 100 System combines complementary metal-oxidesemi conductor (CMOS) technology with the proven accuracy of Illumina sequencing by synthesis (SBS) chemistry to deliver high-accuracy data with fast turnaround times. The iSeq 100 System generates up to 1.2 Gb of data per run in 19 hours and delivers the high resolution and analytical sensitivity needed for detection of rare variants and transcripts.

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iSeq 100 System performance parameters | ||||
Run configuration | Reads passing filter (PF)/run | Output | Quality scores | Run time |
1 × 36 bp | 4M | 144 Mb | > 85% | ~ 9 hours |
1 × 50 bp | 4M | 200 Mb | > 85% | ~ 9 hours |
1 × 75 bp | 4M | 300 Mb | > 80% | ~ 10 hours |
2 × 75 bp | 4M | 600 Mb | > 80% | ~ 13 hours |
2 × 150 bp | 4M | 1.2 Gb | > 80% | ~ 19 hours |
UNIQUE PRODUCT BENEFITS
FAST LIBRARY PREPARATION
A The iSeq 100 System is compatible with the full suite of Illumina library preparation kits. With the Nextera™ XT and Illumina DNA Prep Library Kits, researchers can prepare multiplexed libraries in 3-4 hours for small genome and direct long-range amplicon sequencing. In addition, the AmpliSeq™ for Illumina Targeted Resequencing Solution offers expertly designed content.
WIDE RANGE OF APPLICATIONS
With a maximum output of 2.0 Gb, the iSeq 100System offers rapid, multiplexed sequencing for a range of applications:
- Small whole-genome sequencing
- Targeted resequencing AmpliSeq for Illumina Targeted Resequencing
- Long-range PCR
- De novo sequencing
- Gene editing validation
- Metagenomics (16S rRNA-sequencing)
- Targeted mRNA sequencing
- Small RNA sequencing
- Multiple genome assessment
- Human leukocyte antigen (HLA) sequence based typing
iSeq 100 System applications and run configurations | ||
Application | Samples/run | Run time |
Small genome sequencing 5-10 Mb genomes, 30× coverage 2 × 150 bp | 1-8 | ~ 19 hours |
Targeted gene expression profiling Up to 500 targets 1 × 50 bp | 1-48 | ~ 9 hours |
Targeted amplicon sequencing Up to 3000 amplicons 2 × 250 bp | 1-48 | ~ 19 hours |
EASY, FLEXIBLE DATA ANALYSIS
The iSeq 100 System offers several data analysis options, including onboard and cloud-based solutions. Local Run Manager, a fully integrated onboard analysis software, features modular architecture to support current and future assays. Local Run Manager supports sequencing run planning, library and run tracking with audit trails, and onboard data analysis module integration. While Local Run Manager runs on the instrument computer, users can monitor run progress and view analysis results from remote computers connected to the same network. After a sequencing run is complete, Local Run Manager automatically launches data analysis using one of the application-specific analysis modules.
iSeq 100 System specifications | |
Parameter | Specification |
Instrument configuration | RFID tracking for consumables |
Instrument control computer (Internal)a | Base Unit: Celeron J1900 2 GHz Quad Core CPU Memory: 8 GB RAM Hard Drive: 240 GB SSD Operating System: Windows 10 loT Enterprise |
Operating environment | Temperature: 15°C to 30°C (22.5°C ± 7.5°C) Humidity: noncondensing 20%–80% relative humidity Altitude: Less than 2000 m (6500 ft) Air Quality: pollution degree rating of II Ventilation: up to 2048 BTU/hr @ 600 W For indoor use only |
Light emitting diode (LED) | 520 nm, 1.5 W/cm2 at image plane |
Dimensions | W×D×H (raised monitor): 30.5 cm × 33 cm × 42.5 cm (12.0 in × 13.0 in × 16.7 in) Weight: 16 kg (35 lbs) Crated Weight: 21 kg (47 lbs) |
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