NextSeq 550

NextSeq 550

The NextSeq 550 System combines tried-and-true next-generation sequencing (NGS) and array capabilities with tunable outputs, enabling both small and large labs to scale to meet their needs. As a foundational instrument in the Illumina NGS system portfolio, the NextSeq 550 System is ideal for labs that want to expand beyond their current capacity and new labs interested in harnessing the complementary powers of sequencing and genotyping on a single instrument (Figure 1). Its fast DNA-to-results workflow enables rapid sequencing of exomes, targeted panels, and transcriptomes in a single run, with the flexibility to switch to low- or high-throughput sequencing as needed.

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NextSeq 550 System specifications



Instrument configuration

RFID tracking for consumables

Instrument control computer (Internal)a

Base Unit: Dual Intel Xeon E5-2618L 2.2 GHz CPU Memory: 96 GB RAM

Hard Drive: 750 GB

Operating System: Windows 10 enterprise

Operating environment

Temperature: 19°C to 25°C (22°C ± 3°C)

Humidity: Noncondensing 20%–80% relative humidity Altitude: Less than 2000 m (6500 ft)

Air Quality: Pollution degree rating of II Ventilation: Up to 2048 BTU/hr @ 600 W For Indoor Use Only

Light emitting diode (LED)

520 nm, 650 nm; Laser diode: 780 nm, Class IIIb


W × D × H: 53.3 cm × 63.5 cm × 58.4 cm (21.0 in × 25.0 in

× 23.0 in)

Weight: 83 kg (183 lb)

Crated Weight: 151.5 kg (334 lb)






NextSeq 550 System sequencing performance

Flow cell configuration

Read length


Data quality

High-output flow cell Up to 400M single reads

Up to 800Mpaired-end reads

2 × 150 bp

100-120 Gb

> 75% > Q30

2 × 75 bp

50-60 Gb

> 80% > Q30


1 × 75 bp

25-30 Gb

> 80% > Q30

Mid-output flow cell

Up to 130M single reads

Up to 260M paired-end reads

2 × 150 bp

32-39 Gb

> 75% > Q30

2 × 75 bp

16-19 Gb

> 80% > Q30




The NextSeq 550 System features an intuitive user interface and load-and-go design that allow users to perform various sequencing applications with minimal training and instrument set-up time.


The NextSeq 550 System offers:


Ready-to-use reagents

Radio-frequency identification (RFID)–encoded consumables Integrated cluster generation and sequencing capabilities Easy-to-follow controls

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