MiSeq System
October 6, 2022
NextSeq 550Dx
October 6, 2022
NextSeq 550
NextSeq 550
The NextSeq 550 System combines tried-and-true next-generation sequencing (NGS) and array capabilities with tunable outputs, enabling both small and large labs to scale to meet their needs. As a foundational instrument in the Illumina NGS system portfolio, the NextSeq 550 System is ideal for labs that want to expand beyond their current capacity and new labs interested in harnessing the complementary powers of sequencing and genotyping on a single instrument (Figure 1). Its fast DNA-to-results workflow enables rapid sequencing of exomes, targeted panels, and transcriptomes in a single run, with the flexibility to switch to low- or high-throughput sequencing as needed.
| NextSeq 550 System specifications | |
| Parameter | Specification |
| Instrument configuration | RFID tracking for consumables |
| Instrument control computer (Internal)a | Base Unit: Dual Intel Xeon E5-2618L 2.2 GHz CPU Memory: 96 GB RAM
Hard Drive: 750 GB Operating System: Windows 10 enterprise |
| Operating environment | Temperature: 19°C to 25°C (22°C ± 3°C)
Humidity: Noncondensing 20%–80% relative humidity Altitude: Less than 2000 m (6500 ft) Air Quality: Pollution degree rating of II Ventilation: Up to 2048 BTU/hr @ 600 W For Indoor Use Only |
| Light emitting diode (LED) | 520 nm, 650 nm; Laser diode: 780 nm, Class IIIb |
| Dimensions | W × D × H: 53.3 cm × 63.5 cm × 58.4 cm (21.0 in × 25.0 in
× 23.0 in) Weight: 83 kg (183 lb) Crated Weight: 151.5 kg (334 lb) |
UNIQUE PRODUCT BENEFITS
EXCEPTIONAL SEQUENCING PERFORMANCE
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NextSeq 550 System sequencing performance |
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| Flow cell configuration | Read length | Output | Data quality |
| High-output flow cell Up to 400M single reads
Up to 800Mpaired-end reads |
2 × 150 bp | 100-120 Gb | > 75% > Q30 |
| 2 × 75 bp | 50-60 Gb | > 80% > Q30 | |
| 1 × 75 bp | 25-30 Gb | > 80% > Q30 | |
| Mid-output flow cell
Up to 130M single reads Up to 260M paired-end reads |
2 × 150 bp | 32-39 Gb | > 75% > Q30 |
| 2 × 75 bp | 16-19 Gb | > 80% > Q30 | |
EASY SYSTEM OPERATION
The NextSeq 550 System features an intuitive user interface and load-and-go design that allow users to perform various sequencing applications with minimal training and instrument set-up time.
The NextSeq 550 System offers:
Ready-to-use reagents
Radio-frequency identification (RFID)–encoded consumables Integrated cluster generation and sequencing capabilities Easy-to-follow controls
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