iSeq 100
iSeq 100
Next-generation sequencing (NGS) is easier and more affordable with the compact Illumina iSeq 100 System. The iSeq 100 System combines complementary metal-oxidesemi conductor (CMOS) technology with the proven accuracy of Illumina sequencing by synthesis (SBS) chemistry to deliver high-accuracy data with fast turnaround times. The iSeq 100 System generates up to 1.2 Gb of data per run in 19 hours and delivers the high resolution and analytical sensitivity needed for detection of rare variants and transcripts.
| iSeq 100 System performance parameters | ||||
| Run configuration | Reads passing filter (PF)/run | Output | Quality scores | Run time |
| 1 × 36 bp | 4M | 144 Mb | > 85% | ~ 9 hours |
| 1 × 50 bp | 4M | 200 Mb | > 85% | ~ 9 hours |
| 1 × 75 bp | 4M | 300 Mb | > 80% | ~ 10 hours |
| 2 × 75 bp | 4M | 600 Mb | > 80% | ~ 13 hours |
| 2 × 150 bp | 4M | 1.2 Gb | > 80% | ~ 19 hours |
Features
1-color SBS chemistry.
CMOS detection.
Patterned/ExAmp Flowcell.
Fluidics contained cartridge.
Compact footprint, 1 ft3.
Run time: 17 hrs (300 cycles).
Introduced in 2018.
Benefits
Bacterial, plasmid & viral sequencing (WGS, small genomes).
Targeted regions, amplicon sequencing: 16S metagenomics, Deeplex Myc
tuberculosis sequencing, CovidSeq assay, microbial amplicon Prep.
Mitochondrial DNA sequencing.
Small Amliseq panels: Oncology (BRCA, focus, Cancer HotSpot), Immune
response, custom DNA panel.
Human Leukocyte Antigen (HLA) typing (CareDx).
Library Quality Control.
