The MiniSeq System delivers the quality and reliability of Illumina next-generation sequencing (NGS) technology in a powerful, accessible benchtop sequencer with a small footprint. This small, robust system turns a broad range of NGS methods into approachable, easy-to-use research tools, enabling researchers to take control of their sequencing projects. With the MiniSeq System, there is no need to wait to batch samples for sequencing on a high-throughput instrument; researchers can sequence on demand.
The MiniSeq System is a small, robust benchtop sequencer that enables NGS to become an everyday tool in laboratories worldwide. Incorporating advances in SBS chemistry, the flexible MiniSeq System features push- button operation and streamlined library-to-results workflows that allow researchers to perform popular NGS applications. Its price point and cost-effective operation, even for low numbers of samples, makes the power of proven Illumina sequencing more accessible than ever.
MiniSeq System specifications | |
Parameter | Specification |
Instrument configuration | RFID tracking for consumables |
Instrument control computer (Internal)a | Base unit: Intel Core i7-4700EQ 2.4 GHz CPU Memory: 16 GB DDR3L RAM
Hard drive: 1 Tb Operating system: Windows 10 embedded standard |
Operating environment | Temperature: 19°C to 25°C (22°C ± 3°C)
Humidity: noncondensing 20%–80% relative humidity Altitude: less than 2000 m (6500 ft) Air quality: pollution degree rating of II, air particulate cleanliness levels per ISO9 (ordinary room air) or better Ventilation: up to 2048 BTU/hr @ 600 W For indoor use only |
Light emitting diode (LED) | 515 nm, 650 nm |
Dimensions | W × D × H: 45.6 cm × 48 cm × 51.8 cm (18.0 in × 18.9 in × 20.4 in)
Weight: 45 kg (99 lb) Crated weight: 56.5 kg (125 lb) |
The MiniSeq System features a simple, integrated, library-to-results workflow that enables sequencing of both DNA and RNA with minimal hands-on time. It is ideal for targeted research applications such as cancer sequencing and gene expression profiling.
The system offers cross-method flexibility, enabling an easy transition between sequencing projects for both DNA and RNA applications. Demonstrated and optimized workflows are available for small RNA discovery, targeted resequencing, targeted RNA sequencing, and profiling of solid and hematological tumors.
Flexibility for multiple applications | ||||
Application | High-output reagent kit | Mid-output reagent kit | ||
No. of samples | Run time | No. of samples | Run time | |
Targeted DNA amplicon sequencing 207 amplicons
500× coverage 2 × 150 bp |
96 | 24 hours | 32 | 17 hours |
Targeted expression profiling
65 targets 1 × 50 bp |
384 | 7 hours | 123 | 6 hours |
Enrichment panel 1 Mb region
100× coverage 2 × 75 bp |
23 | 13 hours | 7 | 12 hours |
Viral Pathogen Panel 1M reads/sample
1 × 100 bp (Rapid Kit) |
20 | < 5 hours | N/Ab | N/Ab |
microRNA sequencing 5M reads/sample
1 × 36 bp |
5 | 4 hours | 2 | 4 hours |
Small whole-genome sequencing
5 Mb genome 30× coverage 2 × 150 bp |
50 | 24 hours | 16 | 17 hours |
a. Run times are without indexes
b. N/A, not applicable |
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