The NextSeq 550Dx is a high-throughput, benchtop next-generation sequencing (NGS) system that is FDA-regulated and CE-in vitro diagnostic (IVD) marked. This means that it can be used in clinical laboratories to develop and perform a wide range of applications, from NGS IVD assays using targeted panels to clinical research applications that include methods from targeted panels to whole genomes.
Key features of the NextSeq 550Dx:
- High throughput: The NextSeq 550Dx can generate up to 400 million reads per run, making it a powerful tool for large-scale sequencing projects.
- Accuracy: The NextSeq 550Dx produces highly accurate sequencing data, with a read accuracy of greater than 99%.
- Flexibility: The NextSeq 550Dx can be used to sequence a variety of sample types, including DNA, RNA, and FFPE tissue. It is also compatible with a wide range of sequencing kits.
- Ease of use: The NextSeq 550Dx is designed to be easy to use, even for users with limited NGS experience.
Applications of the NextSeq 550Dx:
- NGS IVD assays using targeted panels: The NextSeq 550Dx can be used to perform a variety of NGS IVD assays using targeted panels. These assays are designed to detect specific genetic variants associated with diseases such as cancer, hereditary disorders, and infectious diseases.
- Clinical research applications: The NextSeq 550Dx can also be used for clinical research applications. These studies may involve sequencing whole genomes, exomes, or targeted panels to identify genetic variants that are associated with disease or to study the effects of treatment.
Benefits of the NextSeq 550Dx:
- Improved patient care: The NextSeq 550Dx can help to improve patient care by enabling clinical laboratories to offer a wider range of NGS IVD assays. These assays can help to diagnose diseases more accurately and quickly, and they can also be used to guide treatment decisions.
- Accelerated research: The NextSeq 550Dx can help to accelerate clinical research by providing researchers with a powerful tool for sequencing large numbers of samples. This can help to identify new genetic variants associated with disease and to develop new treatments.
Overall, the NextSeq 550Dx is a powerful and versatile NGS system that is ideal for a wide range of applications in clinical and research settings. It offers high throughput, accuracy, flexibility, and ease of use.
The NextSeq 550Dx uses a sequencing-by-synthesis (SBS) technology to sequence DNA. SBS is a process by which fluorescently labeled nucleotides are added to a growing DNA strand. The fluorescent labels are detected by a camera, and the sequence of the DNA strand is determined based on the order of the fluorescent labels.
The NextSeq 550Dx is a compact and easy-to-use system. It can be operated by a single user and does not require a dedicated sequencing room. The system is also relatively affordable, making it a good option for clinical laboratories and research institutions of all sizes.
The NextSeq 550Dx is a valuable tool for improving patient care and accelerating clinical research. It is a powerful and versatile NGS system that offers high throughput, accuracy, flexibility, and ease of use.