MiSeq System
The MiSeq System offers the first DNA-to-data sequencing platform integrating cluster generation, amplification, sequencing, and data analysis into a single instrument. Its small footprint—approximately two square feet—fits easily into virtually any laboratory environment. The MiSeq System leverages Illumina sequencing by synthesis (SBS) chemistry, a proven next-generation sequencing (NGS) technology responsible for generating more than 90% of the world’s sequencing data.
| MiSeq System specifications | |
| Parameter | Specification |
| Instrument configuration | RFID tracking for consumables MiSeq Control Software
Local Run Manager Software |
| Instrument control computer (Internal)a | Base Unit: Intel Core i7-2710QE 2.10 GHz CPU Memory: 16 GB RAM
Drive: 1 TB Solid State Drive Operating System: Windows 10 Enterprise LTSC |
| Operating environment | Temperature: 22°C ± 3°C
Humidity: Noncondensing 20%-80% Altitude: Less than 2,000 m (6,500 ft) Air Quality: Pollution degree rating of II Ventilation: Maximum of 1,364 BTU/h For Indoor Use Only |
| Light emitting diode (LED) | 530 nm, 660 nm |
| Dimensions | W × D × H: 68.6 cm × 56.5 cm × 52.3 cm (27.0 in × 22.2 in × 20.6 in)
Weight: 57.2 kg (126 lb) Crated Weight: 93.6 kg (206 lb) |
UNIQUE PRODUCT BENEFITS
SIMPLE, INTUITIVE NGS WORKFLOW
The MiSeq System offers straightforward, easy-to-follow instrument control software. Perform instrument opera- tions with an intuitive touch screen interface, use plug-and-play reagent cartridges with radio frequency identifier (RFID) tracking, consult on-screen video tutorials, and enjoy step-by-step guidance throughout each sequencing workflow.
FAST TURNAROUND TIME
For results in hours rather than days, the combination of rapid library preparation and the MiSeq System delivers a simple, accelerated turnaround time. Prepare your sequencing library in as few as three hours with Illumina DNA Prep library prep reagents, then move to automated clonal amplification, sequencing, and quality-scored base calling in as few as 5.5 hours on the MiSeq System. Sequence alignment can be completed directly on the onboard instrument computer with MiSeq Local Run Manager software or through BaseSpace Sequence Hub within three hours.
MiSeq System
The MiSeq System offers the first DNA-to-data sequencing platform integrating cluster generation, amplification, sequencing, and data analysis into a single instrument. Its small footprint—approximately two square feet—fits easily into virtually any laboratory environment. The MiSeq System leverages Illumina sequencing by synthesis (SBS) chemistry, a proven next-generation sequencing (NGS) technology responsible for generating more than 90% of the world’s sequencing data.
| MiSeq System specifications | |
| Parameter | Specification |
| Instrument configuration | RFID tracking for consumables MiSeq Control Software
Local Run Manager Software |
| Instrument control computer (Internal)a | Base Unit: Intel Core i7-2710QE 2.10 GHz CPU Memory: 16 GB RAM
Drive: 1 TB Solid State Drive Operating System: Windows 10 Enterprise LTSC |
| Operating environment | Temperature: 22°C ± 3°C
Humidity: Noncondensing 20%-80% Altitude: Less than 2,000 m (6,500 ft) Air Quality: Pollution degree rating of II Ventilation: Maximum of 1,364 BTU/h For Indoor Use Only |
| Light emitting diode (LED) | 530 nm, 660 nm |
| Dimensions | W × D × H: 68.6 cm × 56.5 cm × 52.3 cm (27.0 in × 22.2 in × 20.6 in)
Weight: 57.2 kg (126 lb) Crated Weight: 93.6 kg (206 lb) |
Features
4-color SBS chemistry.
Random cluster/bridge amplification.
Run time: 5.5-56 hrs.
Introduced in 2011.
Benefits
Bacterial, plasmid & viral sequencing (WGS, small genomes), Shotgun Metagenomics.
Targeted regions, amplicon sequencing: 16S & ITS metagenomics, Deeplex Myc
tuberculosis sequencing, CovidSeq assay, microbial v3.
Amplicon Prep, Viral Surveillance panel (VSP), AMR enrichments.
Respiratory pathogen detection: RVOP, RPIP (AMR enrichments).
Mitochondrial DNA sequencing.
Targeted gene expression.
Small Amliseq panels: Oncology (BRCA, focus, Cancer HotSpot, Myeloid, Oncoreveal
panels), Immune response, BCR/TCR repertoire, custom DNA panel.
Preimplantation Genetic Testing for Aneuploidy (screening in vitro embryos – VeriSeq,
PGS – Vitrolife).
Human Leukocyte Antigen (HLA) typing (CareDx).
(UPIP).
