MiSeq System

The MiSeq System offers the first DNA-to-data sequencing platform integrating cluster generation, amplification, sequencing, and data analysis into a single instrument. Its small footprint—approximately two square feet—fits easily into virtually any laboratory environment. The MiSeq System leverages Illumina sequencing by synthesis (SBS) chemistry, a proven next-generation sequencing (NGS) technology responsible for generating more than 90% of the world’s sequencing data.

MiSeq System specifications
Instrument configurationRFID tracking for consumables MiSeq Control Software

Local Run Manager Software

Instrument            control computer (Internal)aBase Unit: Intel Core i7-2710QE 2.10 GHz CPU Memory: 16 GB RAM

Drive: 1 TB Solid State Drive

Operating System: Windows 10 Enterprise LTSC

Operating environmentTemperature: 22°C ± 3°C

Humidity: Noncondensing 20%-80% Altitude: Less than 2,000 m (6,500 ft) Air Quality: Pollution degree rating of II Ventilation: Maximum of 1,364 BTU/h

For Indoor Use Only

Light emitting diode (LED)530 nm, 660 nm
DimensionsW × D × H: 68.6 cm × 56.5 cm × 52.3 cm (27.0 in × 22.2 in × 20.6 in)

Weight: 57.2 kg (126 lb)

Crated Weight: 93.6 kg (206 lb)





The MiSeq System offers straightforward, easy-to-follow instrument control software. Perform instrument opera- tions with an intuitive touch screen interface, use plug-and-play reagent cartridges with radio frequency identifier (RFID) tracking, consult on-screen video tutorials, and enjoy step-by-step guidance throughout each sequencing workflow.



For results in hours rather than days, the combination of rapid library preparation and the MiSeq System delivers a simple, accelerated turnaround time. Prepare your sequencing library in as few as three hours with Illumina DNA Prep library prep reagents, then move to automated clonal amplification, sequencing, and quality-scored base calling in as few as 5.5 hours on the MiSeq System. Sequence alignment can be completed directly on the onboard instrument computer with MiSeq Local Run Manager software or through BaseSpace Sequence Hub within three hours.


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