NextSeq 1000 & 2000 System
NextSeq 1000 & 2000 is redesigned from the ground up to maximize future proofing, offering sequencing power for high throughput applications. Offering scalability for evolving needs and larger studies, it supports a vast variety of applications, including Single-Cell Gene Expression, Whole-Exome Sequencing, Shotgun Metagenomics.
| Some of the broader applications available on the NextSeq 1000 and NextSeq 2000 Systems | ||||
| Application | NextSeq 1000 & 2000 P2 Reagents | NextSeq 2000 P3 Reagents | ||
| No. samples | Time | No. samples | Time | |
| Small whole-genome sequencing (300 cycles)
130 Mb genome; > 30× coverage |
30 | ~29 hours | 82 | ~48 hours |
| Whole-exome sequencing (200 cycles)
50× mean targeted coverage; 90% targeted coverage at 20× |
16 | ~21 hours | 44 | ~33 hours |
| Single-cell RNA-Seq (100 cycles)
4K cells, 50K reads/cell |
2 | ~13 hours | 5 | ~19 hours |
| miRNA-Seq or small RNA analysis (50 cycles) 11M reads/sample | 96 | ~11 hours | ||
Push-button DRAGEN informatics pipelines integrated into the NextSeq 1000 and NextSeq 2000 systems
| Pipeline | Applications | Key functionality |
| DRAGEN Enrichment | Whole-exome sequencing Targeted resequencing | Alignment
Small variant calling Somatic variant calling SV/CNV callingb Custom manifest files |
| DRAGEN RNA | Whole-transcriptome gene expression Gene fusion detection | Alignment Fusion detection Gene expression |
| DRAGEN Single-cell RNA | Single-cell whole-transcriptome sequencing | Cell barcodes and error correction Alignment
Gene expression Cell filtering Basic reporting and visualization |
| DRAGEN Compression | Compression of FASTQ files | Lossless compression |
| DRAGEN Germline | Whole-genome sequencing | Alignment
Small variant calling SV/CNV calling |
NextSeq 1000 and NextSeq 2000 specifications
| Specifications |
| Instrument configuration
Self-contained, dry instrument with integrated DRAGEN Bio-IT FPGA secondary analysis |
| Instrument control computer
Base Unit: 2U Microserver located inside the instrument Memory: 288 GB Hard Drive: 3.8 TB SSD Operating System: Linux CentOS 7.6 Operating environment Temperature: 15°C-30°C Humidity: 20%-80% relative humidity, non-condensing Altitude: 0 meters-2000 meters For Indoor Use Only |
| Laser
Wavelengths: 449 nm, 523 nm, 820nm Safety: Class 1 Laser Product |
| Dimensions
W × D × H: 60 cm × 65 cm × 60 cm Weight: 141 kg Crated Dimensions Crated W × D × H: 92 cm × 120 cm × 118 cm Crated Weight: 232 kg |
NextSeq 1000 & 2000
NextSeq 1000 & 2000 is redesigned from the ground up to maximize future proofing, offering sequencing power for high throughput applications. Offering scalability for evolving needs and larger studies, it supports a vast variety of applications, including Single-Cell Gene Expression, Whole-Exome Sequencing, Shotgun Metagenomics.
Features
NextSeq 2000: P1/P2/P3 Flowcells.
NextSeq 1000: P1/P2 Flowcells.
2-color SBS chemistry.
Patterned Flowcell/ExAmp.
Onboard DRAGENTM Bio-IT Platform.
Run time: 12-48 hrs.
Introduced in 2020.
Benefits
Total RNA sequencing.
Single Cell Applications.
Human Whole-Exome Sequencing.
WGS of small/mid-size non-human genomes (plants/animals).
Targeted regions/large panels for Oncology.
WGS of small/mid-size, non-human genomes (plants/animals).
Microbiology/IDT enrichment panels (VSP, UPIP/RPIP/RVOP).
Metagenomic profiling (Shotgun).
COVIDSeqTM Test (RUO).
Genotyping by sequencing (GBS)
| Some of the broader applications available on the NextSeq 1000 and NextSeq 2000 Systems | ||||
| Application | NextSeq 1000 & 2000 P2 Reagents | NextSeq 2000 P3 Reagents | ||
| No. samples | Time | No. samples | Time | |
| Small whole-genome sequencing (300 cycles)
130 Mb genome; > 30× coverage |
30 | ~29 hours | 82 | ~48 hours |
| Whole-exome sequencing (200 cycles)
50× mean targeted coverage; 90% targeted coverage at 20× |
16 | ~21 hours | 44 | ~33 hours |
| Single-cell RNA-Seq (100 cycles)
4K cells, 50K reads/cell |
2 | ~13 hours | 5 | ~19 hours |
| miRNA-Seq or small RNA analysis (50 cycles) 11M reads/sample | 96 | ~11 hours | ||
Push-button DRAGEN informatics pipelines integrated into the NextSeq 1000 and NextSeq 2000 systems
| Pipeline | Applications | Key functionality |
| DRAGEN Enrichment | Whole-exome sequencing Targeted resequencing | Alignment
Small variant calling Somatic variant calling SV/CNV callingb Custom manifest files |
| DRAGEN RNA | Whole-transcriptome gene expression Gene fusion detection | Alignment Fusion detection Gene expression |
| DRAGEN Single-cell RNA | Single-cell whole-transcriptome sequencing | Cell barcodes and error correction Alignment
Gene expression Cell filtering Basic reporting and visualization |
| DRAGEN Compression | Compression of FASTQ files | Lossless compression |
| DRAGEN Germline | Whole-genome sequencing | Alignment
Small variant calling SV/CNV calling |
NextSeq 1000 and NextSeq 2000 specifications
| Specifications |
| Instrument configuration
Self-contained, dry instrument with integrated DRAGEN Bio-IT FPGA secondary analysis |
| Instrument control computer
Base Unit: 2U Microserver located inside the instrument Memory: 288 GB Hard Drive: 3.8 TB SSD Operating System: Linux CentOS 7.6 Operating environment Temperature: 15°C-30°C Humidity: 20%-80% relative humidity, non-condensing Altitude: 0 meters-2000 meters For Indoor Use Only |
| Laser
Wavelengths: 449 nm, 523 nm, 820nm Safety: Class 1 Laser Product |
| Dimensions
W × D × H: 60 cm × 65 cm × 60 cm Weight: 141 kg Crated Dimensions Crated W × D × H: 92 cm × 120 cm × 118 cm Crated Weight: 232 kg |
